Internalin profiling and multilocus sequence typing suggest four Listeria innocua subgroups with different evolutionary distances from Listeria monocytogenes

Table 6 Primers used for MLST

Locus	Putative function	Location^a	Forward primer	Reverse primer	Length (bp)
gyrB	DNA gyrase subunit B	6,031-7,971	TGGTGCATCGGTAGTTAATGC	CAACATCTGGGTTTTCCATCAT	657
dapE	Succinyl diaminopimelate desuccinylase	301,402-302,538	GTAAATATTGATTCGACTAATG	CACTAGCACTTGTTTCACTG	669
hisJ	Histidinol phosphate phosphatase	606,408-607,235	TCCACATGGTACGCATGAT	GGACATGTCAAAATGAAAGATC	714
sigB	Stess responsive alternative sigma factor B	924,734-925,513	CCAAAAGTATCTCAACCTGAT	CATGCATTTGTGATATATCGA	642
ribC	Riboflavin kinaseand FAD synthase	1,364,536-1,365,480	AAGACGATATACTTACATCAT	GTCTTTTTCTAACTGAGCA	633
purM	Phosphoribosyl aminoimidazole synthase	1,893,107-1,894,153	CAAGCTCCACTTTGACAGCTAA	TAAAGCAGGCGTGGACGTA	693
betL	Glycine betaine transporter	2,216,882-2,218,405	ACAGAACATTATCCAAATGAGTT	ACGTTGTGATTTTTTCGGTC	534
gap	Glyceraldehyde 3-phosphate dehydrogenase	2,578,558-2,579,584	CTGGATCAGAAGCTGCTTCCA	GTCGTATTCAAAATGTGGAAGGA	621
tuf	Translation elongation factor	2,816,958-2,818,145	CATTTCTACTCCAGTTACTACT	GCTCTAAACCCCATGTTA	681
Subtotal					5,844

a, Positions correspond to complete genome sequence of L. innocua strain CLIP11262 (AL592022).

ISSN: 1471-2180