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Figure 1 | BMC Microbiology

Figure 1

From: Study of the functionality of the Helicobacter pylori trans-translation components SmpB and SsrA in an heterologous system

Figure 1

Detection of SmpB in E. coli. Detection of SmpB protein in E. coli was performed by western blot with an E. coli SmpB polyclonal antibody. Lane 1: wild type E. coli strain (predicted MW SmpBEc= 18,125 Da), lane 2: ΔsmpB E. coli mutant. Lanes 3-4: SmpBHpdetection in a ΔsmpB E. coli mutant carrying the inducible vector pILL786 expressing the smpBHpgene (predicted MW SmpBHp= 17,682 Da), with or without induction with 1 mM IPTG, respectively. Calibrated amounts of crude bacterial extracts were separated by SDS-15% PAGE. MW: molecular weight.

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