Figure 1From: Study of the functionality of the Helicobacter pylori trans-translation components SmpB and SsrA in an heterologous systemDetection of SmpB in E. coli. Detection of SmpB protein in E. coli was performed by western blot with an E. coli SmpB polyclonal antibody. Lane 1: wild type E. coli strain (predicted MW SmpBEc= 18,125 Da), lane 2: ΔsmpB E. coli mutant. Lanes 3-4: SmpBHpdetection in a ΔsmpB E. coli mutant carrying the inducible vector pILL786 expressing the smpBHpgene (predicted MW SmpBHp= 17,682 Da), with or without induction with 1 mM IPTG, respectively. Calibrated amounts of crude bacterial extracts were separated by SDS-15% PAGE. MW: molecular weight.Back to article page