Distribution of U. diversum in infected HEp-2 cells. LSCM images showing the internalization of U. diversum in HEp-2 cells. Ureaplasmas stained by Dil (in red), actin filaments stained by phalloidin-FITC (green) and cells nuclei stained by TO-PRO-3 (in blue). A and B: Z-series of optical slices (A) and orthogonal projection (B) showing the presence and distribution of ureaplasmas inside HEp-2 cell. C: Image and graphic representation of HEp-2 cells after 12 hours post-infection. The arrow in confocal image indicates the cell in which the ureaplasma (in red) and actin (in green) was analyzed, and the detection of actin and ureaplasmas throughout this cell is represented in the graphic. D: Infected HEp-2 cells submitted to immunofluorescence with anti-lamin antibody (in green), showing ureaplasmas (in red) in the perinuclear region, but not inside the cell nuclei. All the images show ureaplasmas distributed throughout the HEp-2 cytoplasms, and concentrated in the perinuclear region, surrounding the nuclei.