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Figure 5 | BMC Microbiology

Figure 5

From: Genetic analysis of the Staphylococcus epidermidis Macromolecular Synthesis Operon: Serp1129 is an ATP binding protein and sigA transcription is regulated by both σA- and σB-dependent promoters

Figure 5

Primer extension analysis of the S. epidermidis MMSO. Primer extension showing the +1 transcriptional start site (denoted by small arrow) of the (A) P1 promoter upstream of serp1130 using primer 1178, (B) σB-dependent P2 promoter upstream of sigA using primer 1222, and (C) P3 promoter upstream of sigA using primer 1194. WT above each panel represents wildtype S. epidermidis 1457, whereas σBdenotes 1457 sigB::dhfr. (D) Schematic diagram showing the position of proposed promoters (P1, P2, and P3) in the MMSO of S. epidermidis. Small arrows depict the position of the primer extension and RACE primers used to detect the three transcriptional initiation sites. Sequence of putative -35 and -10 boxes, defined transcriptional start site (+1) and ATG start site of (E) P1 promoter, (F) σB-dependent P2 promoter, and (G) P3 promoter.

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