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Table 6 Primer sequences used for transcript expression profiles and gene inactivation

From: The PTS transporters of Lactobacillus gasseri ATCC 33323

Amplified Region

ORF

Primer Sequence

Product Size (bp)

PFK

881

5'-GTTATGGGTCGTGATGTG-3' (F)

85

  

5'-AAGGCTCTTCTGGGATAAC-3' (R)

 

PTS 1

118

5'-TTGGACGTGGCTTAGTTC-3' (F)

85

  

5'-GCACCAGCTACTGTTAAACC-3' (R)

 

PTS 3

149

5'-GATCAGGGCTAGTTGTTG-3' (F)

96

  

5'-CGGCAGCTAAATAACCAC-3' (R)

 

PTS 5

195

5'-GAAGCCTGCGTAAATAAGC-3' (F)

100

  

5'-GTTGCCTGAACAAGTTCC-3' (R)

 

PTS 6

343

5'-CGCAAATGGATACCATGAAAG-3' (F)

77

  

5'-TCCAGTAGTGGTAATCATACG-3' (R)

 

PTS 7

398

5'-GGAATGATGGGAAAGGGAATAG-3' (F)

99

  

5'-TGCATGACCTGTTGGATAAG-3' (R)

 

PTS 8

497

5'-AGCAACCCTATGACTACTACTC-3' (F)

92

  

5'-AGCCATGGTAAGCACTTATC-3' (R)

 

PTS 9

501

5'-CAACTTGTGCGAAGAATTTAAC-3' (F)

95

  

5'-AATTTCAGCAGCTAAGATAACG-3' (R)

 

PTS 10

515

5'-GCTCCAGCTTATGTCGTTAG-3' (F)

109

  

5'-AGAAGCACCAGTTCGAATAG-3' (R)

 

PTS 11

535

5'-GCCAGCCGTTTATGGTATC-3' (F)

82

  

5'-AACAAGGCCACCAAATGC-3' (R)

 

PTS 15

1669

5'-CTTCATTCCGATAGCATGTC-3' (F)

100

  

5'-TGAAGGAGTTGCTGTTGAG-3' (R)

 

PTS 17

1688

5'-TCCAGGTGTCTTGAAAGTAG-3' (F)

99

  

5'-TCAGGGTGAGTGATAATGTC-3' (R)

 

PTS 18

1726

5'-CCATACCACCTACCATAAACAG-3' (F)

91

  

5'-GCTTACGTCTTTGCTTCAG-3' (R)

 

PTS 19

1755

5'-CAATCATTGCACCATACATAGG-3' (F)

78

  

5'-ATTTAGGCGGAATTACAGAAC-3' (R)

 

PTS 20

1778

5'-GCGCTTACCGTATACAAAGG-3' (F)

89

  

5'-TGGTGCCAAAGAGTATTCC-3' (R)

 

PTS 21

1794

5'-GCAGGAATGGCAAGTAATAAAC-3' (F)

86

  

5'-GCTATTGATCGTTGGCAAATG-3' (R)

 

AF_1360Bam

1360

5'-ATGCGGATCC-CGGCAGCCATAGTATATTG-3' (F)

836

AF_1360Nco

 

5'-ATGCCCATGG-TCATGGCTCGTTCATTAG-3' (R)

 

AF_ori+

--

5'-GATAATGAACTGTGCTGATTAC-3' (F)

1071

AF_EI+

 

5'-TGGGTTATATGGTTGGTAAAG-3' (R)

 

AF_ori-

--

5'-TTCAATCGCCAACGAATC-3' (F)

1020

AF_EI-

 

5'-AGTGATACAGCTCAACTTAAC-3' (R)

 

MM_1669Bam

1669

5'-AGTCGGATCCTGCAGCAGGTATGATTAAAG-3' (F)

819

MM_1669Nco

 

5'-AGTCCCATGGAATAGCTGGTTCAGTAACAC-3' (R)

 

AF_ori+

--

5'-GATAATGAACTGTGCTGATTAC-3' (F)

999

MM_PTS15+

 

5'-TGCTGATGACGATTTAGATG-3' (R)

 

AF ori-

--

5'-TTCAATCGCCAACGAATC-3' (F)

1039

MM_PTS15-

 

5'-GCTGCAATACAACTTAAGAC-3' (R)

 

MM_1778Bam

1778

5'-AGTCGGATCCCAGGTTTGTTTGGAGCAAAG' (F)

760

MM_1778Nco

 

5'-AGTCCCATGGTGCTGGTTCAGTAATACCAAG-3' (R)

 

AF_ori+

--

5'-GATAATGAACTGTGCTGATTAC-3' (F)

894

MM_PTS20+

 

5'-CTTAGTAGCTGGTGGTTTG-3' (R)

 

AF ori-

--

5'-TTCAATCGCCAACGAATC-3' (F)

990

MM_PTS20-

 

5'-TACACCTGCACCAATTAAAG-3' (R)

 

MM_1795Bam

1795

5'-AGTCGGATCCAAGGTCCTGATGATATTAGAG' (F)

675

MM_1795Nco

 

5'-AGTCCCATGGATAGCTTTAAGCGCATCTTC-3' (R)

 

AF_ori+

--

5'-GATAATGAACTGTGCTGATTAC-3' (F)

854

MM_PTS21+

 

5'-TAGTCACGGTGGCTTTG-3' (R)

 

AF ori-

--

5'-TTCAATCGCCAACGAATC-3' (F)

895

MM_PTS21-

 

5'-CACTGTAAGCCATGGAAC-3' (R)

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