Table 5 Bacterial strains and plasmids

L. gasseri

NCK334

ATCC 33323, human intestinal isolate

ATCC

MJM79

ATCC 33323 with pTRK669

This study

MJM75

ATCC 33323 EI::pMJM-1, EI^-

This study

MJM99

ATCC 33323 PTS 15::pMJM-4, PTS 15^-

This study

MJM100

ATCC 33323 PTS 20::pMJM-5, PTS 20^-

This study

MJM101

ATCC 33323 PTS 21::pMJM-6, PTS 21^-

This study

NCK100

ADH, human intestinal isolate

[43]

MJM55

ATCC 19992

ATCC

E. coli

EC 1000

RepA⁺ MC1000, Km^r, carrying a single copy of the pWV01 repA gene in the glgB gene; host for pORI28-based plasmids

[44]

NCK1609

EC1000(pORI28)

[44]

NCK1391

EC1000(pTRK669)

[44]

MJM80

EC1000(pMJM-1)

This study

MJM103

EC1000(pMJM-4)

This study

MJM104

EC1000(pMJM-5)

This study

MJM105

EC1000(pMJM-6)

This study

Plasmids

pORI28

Em^r, ori (pWV01), replicates only with repA provided in trans

[44]

pTRK669

ori (pWV01), Cm^r, provides repA in trans, temperature sensitive

[44]

pMJM-1

2.5 kb, pORI28 with 836-bp internal L. gasseri ATCC 33323 EI fragment

This study

pMJM-4

2.5 kb, pORI28 with 819-bp internal L. gasseri ATCC 33323 PTS 15 fragment

This study

pMJM-5

2.4 kb, pORI28 with 760-bp internal L. gasseri ATCC 33323 PTS 20 fragment

This study

pMJM-6

2.3 kb, pORI28 with 675-bp internal L. gasseri ATCC 33323 PTS 21 fragment

This study