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Figure 7 | BMC Microbiology

Figure 7

From: Binding and activation of host plasminogen on the surface of Francisella tularensis

Figure 7

Identification of putative PLG-binding proteins of FT. Sarkosyl-soluble and insoluble protein fractions of FTLVS were separated by SDS-PAGE and transferred to PVDF membrane. Membranes were then blotted with huPLG (3 ug/mL) followed by anti-PLG antibody and HRP-conjugated secondary antibody to detect PLG-binding proteins (Panel A). Protein bands on an identical Coomassie Blue-stained SDS-PAGE gel corresponding to those identified via blotting (Panel B) were excised and identified using proteomic methodologies (Panel C).

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