Table 4 List of primers used for the quantification of gene expression by real time RT PCR.
primer | sequence 5'-3' | length (bp) | Reference |
|---|---|---|---|
TNFαFor | CATCTTCTCAAAATTCGAGTGACAA | 175 | [34] |
TNFαRev | TGGGAGTAGACAAGGTACAACCC | ||
IL-12p40For | GGAAGCACGGCAGCAGAATA | 180 | [34] |
IL-12p40Rev | AACTTGAGGGAGAAGTAGGAATGG | ||
IL-18For | CAGGCCTGACATCTTCTGCAA | 105 | [34] |
IL-18Rev | TCTGACATGGCAGCCATTGT | ||
IFNγFor | AACAGCAAGGCGAAAAAGGA | 92 | this study |
IFNγRev | GTGGACCACTCGGATGAGC | ||
iNOSFor | CAGCTGGGCTGTACAAACCTT | 95 | [34] |
iNOSRev | CATTGGAAGTGAAGCGTTTCG | ||
β-actinFor | CTTTGCAGCTCCTTCGTTG | 150 | this study |
β-actinRev | ACGATGGAGGGGAATACAGC |