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Table 1 Bacterial composition of cheese surface consortium F by a culture dependent method1

From: Population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis

Bacterial species   Accession number2 Similarity (%) Isolation media3 Viable count [CFU cm-2] Percentage on TGYA
Brevibacterium linens
(or Brevibacterium aurantiacum4)
TGYA 7.5.108 32.5%
Staphylococcus vitulinus   GenBank:NR_024670 99.6 TGYA 6.0.108 26.0%
Brachybacterium tyrofermentans   GenBank:X91657 97.9 TGYA 4.5.108 19.5%
Corynebacterium casei   GenBank:DQ361013 100.0 TGYA 1.5.108 6.5%
Microbacterium gubbeenense   GenBank:AF263564 97.9 TGYA 1.5.108 6.5%
Marinilactibacillus psychrotolerans   GenBank:AB083413 99.8 TGYA 1.5.108 6.5%
Brachybacterium sp.   GenBank:AF513397 99.9 TGYA 0.7.108 3.0%
Staphylococcus equorum   GenBank:NR_027520 98.8-99.1 MSA 3.0.108 -
Staphylococcus epidermidis   GenBank:NC_004461 98.5 MSA 8.107 -
Facklamia tabacinasalis   GenBank:Y17820 99.1 BP 6.105 -
Lactococcus lactis   GenBank:NC_002662 99.5 MRS 4.104 -
Enterococcus devriesei   GenBank:AJ891167 98.2 MRS 1.104 -
Enterococcus malodoratus   GenBank:Y18339 99.8 MRS 2.103 -
Enterococcus faecalis   GenBank:AJ420803 99.3 KFS 2.102 -
Enterococcus faecium   GenBank:EU547780 100.0 KFS 6.101 -
  1. 1 128 isolates, i.e. ca. 25 isolates per media, were analyzed by TTGE and grouped into identical TTGE profiles. A representative isolate of each profile was identified by 16S rDNA sequencing. After the assignment of all isolates to a species, the percentage of each species on each of the five media was assessed. The cell count of a species was calculated by multiplying percentage and cell count determined for the corresponding media.
  2. 2 Closest 16S rDNA sequence in the GenBank public database
  3. 3 Total cell count was determined on TGYA. In addition, staphylococci were enumerated on BP agar and MSA, lactic acid bacteria on MRS agar and enterococci on KFS agar.
  4. 4 Given the polymorphy in the intraspecies diversity of B. linens (Oberreuter et al. [52]), strain assignation to B. linens or the related species B. aurantiacum based on 16S rDNA analysis only was considered not reliable.