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Table 1 Results of fluorescence in situ hybridization of all Francisella (F.) tularensis and F. philomiragia strains used in this study.

From: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA

Species and origin

Strain

Alt. designation

Hybridization probe

   

Bwall 1448

Bwphi 1448

Bwhol 1151

Bwnov 168

Bwtume 168II

Bwmed 1397

F. tul. subsp. tularensis

        

   Human, Ohio, 1941

FSC237

Schu S4

+

-

-

-

+

-

   Squirrel, Georgia (USA)

FSC033

SnMF

+

-

-

-

+

-

   Tick, BC, Canada, 1935

FSC041

Vavenby

+

-

-

-

+

-

   Canada

FSC042

Utter

+

-

-

-

+

-

   Hare Nevada, 1953

FSC054

Nevada 14

+

-

-

-

+

-

   Human, Utah, 1920

FSC230

ATCC 6223

+

-

-

-

+

-

F. tul. subsp. holarctica

        

Live vaccine strain,

Russia

F49

ATCC 29684

+

-

+

-

-

-

   Origin unknown

F1

BGA

+

-

+

-

-

-

   Human, Austria, 1997

F2

 

+

-

+

-

-

-

Human, Austria (Wien),

1997

F11

 

+

-

+

-

-

-

Hare, Austria, 1994

(4 isolates)

F6-7,

F54-55

 

+

-

+

-

-

-

   Hare, Austria, 1995

F8

 

+

-

+

-

-

-

   Hare, Austria, 1996

F9

 

+

-

+

-

-

-

Hare, Austria, 1997

(13 isolates)

F12-F24

 

+

-

+

-

-

-

Hare, Austria, 1998

(2 isolates)

F46, F47

 

+

-

+

-

-

-

Macaque, Germany, 2005

(4 isolates)

F 101,

F107-109

 

+

-

+

-

-

-

Water vole, Germany,

2005, (2 isolates)

F105-106

 

+

-

+

-

-

-

F. tul. subsp. mediasiatica

        

Miday gerbil, Kazakhstan, 1965

F63

FSC147

+

-

-

-

+

+

Tick, Central Asia, 1982

F64

FSC148

+

-

-

-

+

+

Hare, Central Asia, 1965

F65

FSC149

+

-

-

-

+

+

F. tul. subsp. novicida

        

Water, Utah, 1950

F48

ATCC 15482

+

-

-

+

-

-

Human, UK, 2003

F58

FSC595

+

-

-

+

-

-

Human, Texas, 1991

F59

FSC156

+

-

-

+

-

-

Human, Texas, 1995

F60

FSC159

+

-

-

+

-

-

Spain (novicida-like)

F62

FSC454

+

-

-

+

-

-

F. philomiragia

        

Water, Utah

F50

ATCC 25016

+1

+

-

-

-

-

Muskrat, Utah, 1959

F51

ATCC 25015

+1

+

-

-

-

-

Water, Utah, 1960

F93

ATCC 25017

+1

+

-

-

-

-

Water, Utah, 1960

F94

ATCC 25018

+1

+

-

-

-

-

  1. Hybridization with specific probes was only performed if prepared cells had shown a positive signal with probe EUB388 (positive control) and no staining with probe NON-EUB388 (negative control).
  2. +: positive signal; - negative signal with 6-FAM or Cy3-labeld probes
  3. +1: Depending on the formamide concentration used for hybridization. For details refer to Results section.n