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Table 1 Results of fluorescence in situ hybridization of all Francisella (F.) tularensis and F. philomiragia strains used in this study.

From: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA

Species and origin Strain Alt. designation Hybridization probe
    Bwall 1448 Bwphi 1448 Bwhol 1151 Bwnov 168 Bwtume 168II Bwmed 1397
F. tul. subsp. tularensis         
   Human, Ohio, 1941 FSC237 Schu S4 + - - - + -
   Squirrel, Georgia (USA) FSC033 SnMF + - - - + -
   Tick, BC, Canada, 1935 FSC041 Vavenby + - - - + -
   Canada FSC042 Utter + - - - + -
   Hare Nevada, 1953 FSC054 Nevada 14 + - - - + -
   Human, Utah, 1920 FSC230 ATCC 6223 + - - - + -
F. tul. subsp. holarctica         
Live vaccine strain,
Russia
F49 ATCC 29684 + - + - - -
   Origin unknown F1 BGA + - + - - -
   Human, Austria, 1997 F2   + - + - - -
Human, Austria (Wien),
1997
F11   + - + - - -
Hare, Austria, 1994
(4 isolates)
F6-7,
F54-55
  + - + - - -
   Hare, Austria, 1995 F8   + - + - - -
   Hare, Austria, 1996 F9   + - + - - -
Hare, Austria, 1997
(13 isolates)
F12-F24   + - + - - -
Hare, Austria, 1998
(2 isolates)
F46, F47   + - + - - -
Macaque, Germany, 2005
(4 isolates)
F 101,
F107-109
  + - + - - -
Water vole, Germany,
2005, (2 isolates)
F105-106   + - + - - -
F. tul. subsp. mediasiatica         
Miday gerbil, Kazakhstan, 1965 F63 FSC147 + - - - + +
Tick, Central Asia, 1982 F64 FSC148 + - - - + +
Hare, Central Asia, 1965 F65 FSC149 + - - - + +
F. tul. subsp. novicida         
Water, Utah, 1950 F48 ATCC 15482 + - - + - -
Human, UK, 2003 F58 FSC595 + - - + - -
Human, Texas, 1991 F59 FSC156 + - - + - -
Human, Texas, 1995 F60 FSC159 + - - + - -
Spain (novicida-like) F62 FSC454 + - - + - -
F. philomiragia         
Water, Utah F50 ATCC 25016 +1 + - - - -
Muskrat, Utah, 1959 F51 ATCC 25015 +1 + - - - -
Water, Utah, 1960 F93 ATCC 25017 +1 + - - - -
Water, Utah, 1960 F94 ATCC 25018 +1 + - - - -
  1. Hybridization with specific probes was only performed if prepared cells had shown a positive signal with probe EUB388 (positive control) and no staining with probe NON-EUB388 (negative control).
  2. +: positive signal; - negative signal with 6-FAM or Cy3-labeld probes
  3. +1: Depending on the formamide concentration used for hybridization. For details refer to Results section.n