Figure 6From: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNASpecific detection of F. tularensis subsp. holarctica in a liver tissue sample (mouse) fixed in formalin and embedded in paraffin for more than four years. After deparaffinization and fluorescence in situ hybridization, bacterial cells can be visualized in small granuloma (A: phase contrast microscopy; B: fluorescence microscopy, DAPI staining; C: Specific staining of F. tularensis subsp. holarctica).Back to article page