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Figure 4 | BMC Microbiology

Figure 4

From: Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA

Figure 4

Left: Artificial mixture of F. tularensis subsp. tularensis (Schu S4, green circle) and F. tularensis subsp. mediasiatica (FSC 148, red circle), phase contrast microscopy. Right: Fluorescence microscopy after hybridization with probes Bwmed1379-Cy3 and Bwtume168II-6-FAM with 20% formamide. F. tularensis subsp. tularensis cells only bind to probe Bwtume168II-6-FAM (green fluorescence) whereas bacterial cells of F. tularensis subsp. mediasiatica bind to both probes resulting in a yellow-orange fluorescence.

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BMC Microbiology

ISSN: 1471-2180

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