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Table 1 Bacterial strains and plasmids.

From: The Sinorhizobium meliloti RNA chaperone Hfq influences central carbon metabolism and the symbiotic interaction with alfalfa

Strain/Plasmid Relevant characteristics Reference/Source
Bacteria   
S. meliloti   
   1021 Wild-type SU47 derivative, Smr [75]
   2011 Wild-type SU47 derivative, Smr [76]
   1021Δhfq 1021 hfq mutant strain, Smr This work
   2011-1.2 2011 hfq insertion derivative (control str.), Smr, Kmr This work
   2011-3.4 2011 hfq insertion mutant, Smr, Kmr This work
   1021hfqFLAG 1021 derivative expressing a 3 × Flag-tagged Hfq, Smr This work
E. coli   
   DH5α F- endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupG, φ80d, lacZ ΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ- Bethesda Research Lab.
Plasmids   
   pRK2013 Helper plasmid, Col E1, Kmr [77]
   pGEM®-T Easy Cloning vector for PCR, Apr Promega Corporation
   pK18mobsacB Suicide vector in S. meliloti Kmr, sacB, ori V [78]
   pJB3Tc19 Broad host-range IncP cloning vector, Apr, Tcr [79]
   pBluescriptII KS+ Multicopy cloning vector, Apr Stratagene
   pGEMhfq 1,684-bp of hfq genomic region in pGEM-T This work
   pK18_1.2 Internal fragment of hfq ORF in pK18mobsacB This work
   pK18_3.4 Internal fragment of hfq ORF in pK18mobsacB This work
   pGEMΔhfq Genomic region with deletion of hfq in pGEM-T This work
   pK18Δhfq Genomic region with deletion of hfq in pK18 This work
   pGEMHfq pGEM-T containing hfq promoter and ORF This work
   pJBHfq pJB3Tc20 containing hfq promoter and ORF This work
   pKS3 × Flag 3 × FLAG encoding fragment in pBluescriptII KS+ This work
   pKS3 × Flag5 pKS3 × Flag with upstream region of hfq This work
   pKSHfq3 × Flag pBluescriptII KS+ with Flag-tagged hfq ORF This work
   pGEMHfq3 × Flag pGEM-T with Flag-tagged hfq ORF This work
   pK18Hfq3 × Flag pK18mobsacB with Flag-tagged hfq ORF This work