Figure 6
Hfq contributes to the regulation of nifA and fixK expression. RT-PCR analysis on RNA extracted from the wild-type strain 1021 (lanes 1 and 3) and the hfq mutant (lanes 2 and 4) before (lanes 1 and 2) and after (lanes 3 and 4) culture incubation for 4 h in microaerobiosis (2% O2). 16S was amplified as constitutive control of expression. Mock-treated (no RT) RNA samples were also PCR amplified with the same primer combinations to check for absence of DNA contamination (not shown).