Primer | Sequence (5'-3') | Position | Target gene | Reference |
---|---|---|---|---|
Endpoint PCR | ||||
A189 amoA2R' | GGHGACTGGGAYTTCTGG CCTCKGSAAAGCCTTCTTC | 151-168 802-820 | amoA | |
HAO1F HAO1R | TCAACATAGGCACGGTTCATCGGA ATTTGCCGAACGTGAATCGGAACG | 203-226 1082-1105 | hao | [38] |
NirK1F NirK1R | TGCTTCCGGATCAGCGTCATTAGT AGTTGAAACCGATGTGGCCTACGA | 31-54 809-832 | nirK | [38] |
NorB1F NorB1R | CGGCACTGATGTTCCTGTTTGCTT AGCAACCGCATCCAGTAGAACAGA | 479-502 1215-1238 | norB | [38] |
KNO50F KNO51R | TNANACATGCAAGTCGAICG GGYTACCTTGTTACGACTT | 49-68 1492-1510 | Eubacterial 16S rRNA gene | [39] |
Quantitative PCR | ||||
amoAFq amoARq | GGACTTCACGCTGTATCTG GTGCCTTCTACAACGATTGG | 408-426 524-543 | amoA | [15] |
HAO1Fq HAO1Rq | TGAGCCAGTCCAACGTGCAT AAGGCAACAACCCTGCCTCA | 266-285 331-350 | hao | [38] |
NirK1Fq NirK1Rq | TGCAGGGCATACTGGACGTT AGGTGAACGGGTGCGCATTT | 182-201 291-310 | nirK | [38] |
NorB1Fq NorB1Rq | ACACAAATCACTGCCGCCCA TGCAGTACACCGGCAAAGGT | 958-977 1138-1157 | norB | [38] |
EUBF EUBR | TCCTACGGGAGGCAGCAGT GGACTACCAGGGTATCTAATCCTGTT | 339-357 780-805 | Eubacterial 16S rRNA gene | [34] |