Table 1 Endpoint and real-time PCR primers employed in this study
| Primer | Sequence (5'-3') | Position | Target gene | Reference |
|---|---|---|---|---|
| Endpoint PCR | ||||
|
A189 amoA2R' |
GGHGACTGGGAYTTCTGG CCTCKGSAAAGCCTTCTTC |
151-168 802-820 | amoA | [36, 37] |
|
HAO1F HAO1R |
TCAACATAGGCACGGTTCATCGGA ATTTGCCGAACGTGAATCGGAACG |
203-226 1082-1105 | hao | [38] |
|
NirK1F NirK1R |
TGCTTCCGGATCAGCGTCATTAGT AGTTGAAACCGATGTGGCCTACGA |
31-54 809-832 | nirK | [38] |
|
NorB1F NorB1R |
CGGCACTGATGTTCCTGTTTGCTT AGCAACCGCATCCAGTAGAACAGA |
479-502 1215-1238 | norB | [38] |
|
KNO50F KNO51R |
TNANACATGCAAGTCGAICG GGYTACCTTGTTACGACTT |
49-68 1492-1510 | Eubacterial 16S rRNA gene | [39] |
| Quantitative PCR | ||||
|
amoAFq amoARq |
GGACTTCACGCTGTATCTG GTGCCTTCTACAACGATTGG |
408-426 524-543 | amoA | [15] |
|
HAO1Fq HAO1Rq |
TGAGCCAGTCCAACGTGCAT AAGGCAACAACCCTGCCTCA |
266-285 331-350 | hao | [38] |
|
NirK1Fq NirK1Rq |
TGCAGGGCATACTGGACGTT AGGTGAACGGGTGCGCATTT |
182-201 291-310 | nirK | [38] |
|
NorB1Fq NorB1Rq |
ACACAAATCACTGCCGCCCA TGCAGTACACCGGCAAAGGT |
958-977 1138-1157 | norB | [38] |
|
EUBF EUBR |
TCCTACGGGAGGCAGCAGT GGACTACCAGGGTATCTAATCCTGTT |
339-357 780-805 | Eubacterial 16S rRNA gene | [34] |