Strategies of Nitrosomonas europaea 19718 to counter low dissolved oxygen and high nitrite concentrations

BMC Microbiology

Table 1 Endpoint and real-time PCR primers employed in this study

Primer	Sequence (5'-3')	Position	Target gene	Reference
Endpoint PCR
A189 amoA2R'	GGHGACTGGGAYTTCTGG CCTCKGSAAAGCCTTCTTC	151-168 802-820	amoA	[36, 37]
HAO1F HAO1R	TCAACATAGGCACGGTTCATCGGA ATTTGCCGAACGTGAATCGGAACG	203-226 1082-1105	hao	[38]
NirK1F NirK1R	TGCTTCCGGATCAGCGTCATTAGT AGTTGAAACCGATGTGGCCTACGA	31-54 809-832	nirK	[38]
NorB1F NorB1R	CGGCACTGATGTTCCTGTTTGCTT AGCAACCGCATCCAGTAGAACAGA	479-502 1215-1238	norB	[38]
KNO50F KNO51R	TNANACATGCAAGTCGAICG GGYTACCTTGTTACGACTT	49-68 1492-1510	Eubacterial 16S rRNA gene	[39]
Quantitative PCR
amoAFq amoARq	GGACTTCACGCTGTATCTG GTGCCTTCTACAACGATTGG	408-426 524-543	amoA	[15]
HAO1Fq HAO1Rq	TGAGCCAGTCCAACGTGCAT AAGGCAACAACCCTGCCTCA	266-285 331-350	hao	[38]
NirK1Fq NirK1Rq	TGCAGGGCATACTGGACGTT AGGTGAACGGGTGCGCATTT	182-201 291-310	nirK	[38]
NorB1Fq NorB1Rq	ACACAAATCACTGCCGCCCA TGCAGTACACCGGCAAAGGT	958-977 1138-1157	norB	[38]
EUBF EUBR	TCCTACGGGAGGCAGCAGT GGACTACCAGGGTATCTAATCCTGTT	339-357 780-805	Eubacterial 16S rRNA gene	[34]

ISSN: 1471-2180