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Table 1 Summary of Gene Ontology categories of overrepresented proteins whose expressions increase during polyP deficiency in Pseudomonas sp. B4.

From: New structural and functional defects in polyphosphate deficient bacteria: A cellular and proteomic study

GO Term Annotation Spot Protein Name
IPR
NCBI Accession Theo.
Mr (kDa)/PI
Exp.
Mr (kDa)/PI
Species/Coverage Mascot Score
Biological Process
Protein folding
GO:0006457
1 e, l Trigger factor
IPR008881
gi: 145575278 48.3/4.78 55/5.1 Pseudomonas mendocina ymp/44% 1359
  2 e, l GrpE nucleotide exchange factor
IPR000740
gi: 60549562 20.4/4.9 24/5.1 Pseudomonas putida/29% 267
  3 st, a Chaperonin GroEL
IPR012723
gi: 146308703 56.8/5.02 55/5.2 Pseudomonas mendocina ymp/35% 674
Tricarboxylic acid cycle
GO:0006099
4 e, l Aconitase
IPR004406
gi: 145575802 94.2/5.24 95/5.8 Pseudomonas mendocina ymp/32% 1715
  5 e, l Isocitrate dehydrogenase, NADP-dependent
IPR004436
gi: 146307420 82.1/5.63 90/6.3 Pseudomonas mendocina ymp/24% 1130
Metabolic process
GO:0008152
6 e, l Succinyl-CoA synthetase
IPR005809
gi: 146307523 41.8/5.5 49/6.5 Pseudomonas mendocina ymp/34% 654
ATP synthesis proton transport
GO:0015986
7 st, a ATP synthase F1, delta subunit
IPR000711
gi: 146309623 19/5.87 20/5.6 Pseudomonas mendocina ymp/40% 310
Fatty acid metabolic process
GO:0006631
8 st, l Fatty acid oxidation complex
IPR006180
gi: 146306611 77.5/5.58 70/6.5 Pseudomonas mendocina ymp/51% 159
Metabolic process
GO:0008152
9 st, l Enoyl-CoA hydratase
IPR001753
gi: 146307097 29.8/5.67 27/6.3 Pseudomonas mendocina ymp/54% 61
Fatty acid biosynthetic process
GO:0006633
10 st, l Hydroxymyristoyl-(ACP) dehydratase
IPR010084
gi: 146308063 16.8/6.3 15/7.5 Pseudomonas mendocina ymp/67% 106
  11 st, a Acetyl-CoA carboxylase biotin carboxyl carrier
IPR001249
gi: 26987297 16.2/4.95 20/4.8 Pseudomonas putida KT2440/20% 415
Cysteine biosynthetic process serine
GO:0006535
12 st, l Cysteine synthase
IPR005859
gi: 146306821 34.4/5.89 37/6.5 Pseudomonas mendocina ymp/32% 451
Amino acid biosynthetic process
GO:0008652
13 st, l Aspartate-semialdehyde dehydrogenase
IPR012280
gi: 146307742 40.5/5.33 40/6 Pseudomonas mendocina ymp/63% 128
Branched chain family amino acid biosynthetic process
GO:0009082
14 st, l Dihydroxy-acid dehydratase
IPR004404
gi: 146309219 66.2/5.69 60/6.5 Pseudomonas mendocina ymp/44% 114
Translational elongation
GO:0006414
15 e, l Elongation factor Tu
IPR004541
gi: 146308925 43.9/5.38 45/5.8 Pseudomonas mendocina ymp/43% 847
  16 st, a Elongation factor Ts
IPR001816
gi: 146308073 30.5/5.22 30/5.2 Pseudomonas mendocina ymp/52% 895
Molecular function
ATP binding
GO:0005524
17 e, l ATPase AAA-2 gi: 146308654 95/5.32 90/5.9 Pseudomonas mendocina ymp/40% 2404
Antioxidant activity
GO:0016209
18 e, l Alkyl hydroperoxide reductase
IPR000866
gi: 119860085 17.6/5.02 17/5.1 Pseudomonas putida W619/24% 149
  1. GO: Gene Ontology Term Annotation; Spot numbers correspond to spots in 2D-PAGE; Growth Phase (e:exponential; st: stationary); Culture Medium LB (l: liquid; a: agar plate); IPR: InterPro entry; NCBI accession number from NCBI database; Theo. Mr (kDa)/PI: theoretical molecular mass and isoelectric point; Exp. Mr (kDa)/PI, experimental molecular mass and isoelectric point estimated from the 2D-PAGE gels.