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Table 1 Summary of Gene Ontology categories of overrepresented proteins whose expressions increase during polyP deficiency in Pseudomonas sp. B4.

From: New structural and functional defects in polyphosphate deficient bacteria: A cellular and proteomic study

GO Term Annotation

Spot

Protein Name

IPR

NCBI Accession

Theo.

Mr (kDa)/PI

Exp.

Mr (kDa)/PI

Species/Coverage

Mascot Score

Biological Process

Protein folding

GO:0006457

1 e, l

Trigger factor

IPR008881

gi: 145575278

48.3/4.78

55/5.1

Pseudomonas mendocina ymp/44%

1359

 

2 e, l

GrpE nucleotide exchange factor

IPR000740

gi: 60549562

20.4/4.9

24/5.1

Pseudomonas putida/29%

267

 

3 st, a

Chaperonin GroEL

IPR012723

gi: 146308703

56.8/5.02

55/5.2

Pseudomonas mendocina ymp/35%

674

Tricarboxylic acid cycle

GO:0006099

4 e, l

Aconitase

IPR004406

gi: 145575802

94.2/5.24

95/5.8

Pseudomonas mendocina ymp/32%

1715

 

5 e, l

Isocitrate dehydrogenase, NADP-dependent

IPR004436

gi: 146307420

82.1/5.63

90/6.3

Pseudomonas mendocina ymp/24%

1130

Metabolic process

GO:0008152

6 e, l

Succinyl-CoA synthetase

IPR005809

gi: 146307523

41.8/5.5

49/6.5

Pseudomonas mendocina ymp/34%

654

ATP synthesis proton transport

GO:0015986

7 st, a

ATP synthase F1, delta subunit

IPR000711

gi: 146309623

19/5.87

20/5.6

Pseudomonas mendocina ymp/40%

310

Fatty acid metabolic process

GO:0006631

8 st, l

Fatty acid oxidation complex

IPR006180

gi: 146306611

77.5/5.58

70/6.5

Pseudomonas mendocina ymp/51%

159

Metabolic process

GO:0008152

9 st, l

Enoyl-CoA hydratase

IPR001753

gi: 146307097

29.8/5.67

27/6.3

Pseudomonas mendocina ymp/54%

61

Fatty acid biosynthetic process

GO:0006633

10 st, l

Hydroxymyristoyl-(ACP) dehydratase

IPR010084

gi: 146308063

16.8/6.3

15/7.5

Pseudomonas mendocina ymp/67%

106

 

11 st, a

Acetyl-CoA carboxylase biotin carboxyl carrier

IPR001249

gi: 26987297

16.2/4.95

20/4.8

Pseudomonas putida KT2440/20%

415

Cysteine biosynthetic process serine

GO:0006535

12 st, l

Cysteine synthase

IPR005859

gi: 146306821

34.4/5.89

37/6.5

Pseudomonas mendocina ymp/32%

451

Amino acid biosynthetic process

GO:0008652

13 st, l

Aspartate-semialdehyde dehydrogenase

IPR012280

gi: 146307742

40.5/5.33

40/6

Pseudomonas mendocina ymp/63%

128

Branched chain family amino acid biosynthetic process

GO:0009082

14 st, l

Dihydroxy-acid dehydratase

IPR004404

gi: 146309219

66.2/5.69

60/6.5

Pseudomonas mendocina ymp/44%

114

Translational elongation

GO:0006414

15 e, l

Elongation factor Tu

IPR004541

gi: 146308925

43.9/5.38

45/5.8

Pseudomonas mendocina ymp/43%

847

 

16 st, a

Elongation factor Ts

IPR001816

gi: 146308073

30.5/5.22

30/5.2

Pseudomonas mendocina ymp/52%

895

Molecular function

ATP binding

GO:0005524

17 e, l

ATPase AAA-2

gi: 146308654

95/5.32

90/5.9

Pseudomonas mendocina ymp/40%

2404

Antioxidant activity

GO:0016209

18 e, l

Alkyl hydroperoxide reductase

IPR000866

gi: 119860085

17.6/5.02

17/5.1

Pseudomonas putida W619/24%

149

  1. GO: Gene Ontology Term Annotation; Spot numbers correspond to spots in 2D-PAGE; Growth Phase (e:exponential; st: stationary); Culture Medium LB (l: liquid; a: agar plate); IPR: InterPro entry; NCBI accession number from NCBI database; Theo. Mr (kDa)/PI: theoretical molecular mass and isoelectric point; Exp. Mr (kDa)/PI, experimental molecular mass and isoelectric point estimated from the 2D-PAGE gels.