Development of O-antigen gene cluster-specific PCRs for rapid typing six epidemic serogroups of Leptospira in China

Table 1 PCR primers targeting the specific genes

Serogroup	Target gene	Primer pairs (5'→3')	Annealing temperature(°C)	Amplicon size(bp)
Autumnalis	hypothetical protein	aut-F: TTT TGA TGG GCA TAC TGA	56	298
		aut-R: TAT GCC CTA AGT GAG TTG C
Canicola	dTDP-4-dehydrorhamnose reductase	can-F: CAA AGG TGA TTC ACA AGG	60	341
		can-R: TCA GTG CAT TAG CCG TAT
Grippotyphosa	glycosyltransferase	gri-F: AGA GCC GGA GGA CAG TAA	63	352
		gri-R: CGA TGG GAA ACC AAG GAT
Hebdomadis	carbamoyl transferase	heb-F: GAT TTG ATA AGG CGA AGA	56	656
		heb-R: AAG CTC CAA TAC ATA AGG AC
Icterohaemorrhagiae	glycosyltransferase	ict-F: TTT CAT ACG TTG CGC TTA C	57	590
		ict-R: ATA AAG TCC AGC ATC ATC CA
Sejroe	dehydrogenase	sej-F: CGA CCG AGA TTG ACT ATG TT	60	319
		sej-R: GAA AGC AGC ATA AGT CCC

ISSN: 1471-2180