Figure 5

Formations of F. alocis in carrier-borne biofilms. FISH on different carriers with GAP biofilms using the probes EUB 338-Cy5 (magenta) and FIAL-Cy3 (bright orange) along with DAPI staining (blue). EUB 338 detects the whole bacterial population while FIAL visualizes F. alocis specifically. DAPI stains both bacteria and host cell nuclei. High magnifications show F. alocis in different areas of the biofilms. (a): Overlay of Cy3, Cy5 and DAPI filter sets. In some regions of the biofilm Filifactor rods can reach a considerable length. (b and c): Overlay of Cy3 and DAPI filter sets. (b) shows the radial orientation of F. alocis and other organisms on the surface of a mushroom-like protuberance of the biofilm. (c) shows F. alocis forming test-tube-brush-like structures around a signal-free channel. (d): Overlay of Cy3 and Cy5 filter sets. F. alocis and fusiform bacteria form concentrical structures.