Table 1 Primers used for real-time RT PCR
From: Modulation of iron homeostasis in macrophages by bacterial intracellular pathogens
Gene | Accession number | Forward primer (5' → 3') | Reverse Primer (5' → 3') |
|---|---|---|---|
GAPDH | NM_008084 | AGGTGACACTATAGAATACCCACTAACATCAAATGGGG | GTACGACTCACTATAGGGACCTTCCACAATGCCAAAGTT |
IRP1 | NM_007386 | AGGTGACACTATAGAATAACTTTGAAAGCTGCCTTGGA | GTACGACTCACTATAGGGACTCCACTTCCAGGAGACAGG |
IRP2 | NM_022655 | AGGTGACACTATAGAATATGAAGAAACGGACCTGCTCT | GTACGACTCACTATAGGGAGCTCACATCCAACCACCTCT |
TfR1 | BC054522 | AGGTGACACTATAGAATATGCAGAAAAGGTTGCAAATG | GTACGACTCACTATAGGGATGAGCATGTCCAAAGAGTGC |
Dmt1 | NM_008732 | AGGTGACACTATAGAATAGCCAGCCAGTAAGTTCAAGG | GTACGACTCACTATAGGGAGCTGTCCAGGAAGACCTGAG |
LcnR | NM_021551 | AGGTGACACTATAGAATAGCAAGGCTACCCCATACAAA | GTACGACTCACTATAGGGAAAGAGCGAGGTCTGGGAAAT |
Lcn2 | NM_008491 | AGGTGACACTATAGAATACTGAATGGGTGGTGAGTGTG | GTACGACTCACTATAGGGATATTCAGCAGAAAGGGGACG |
Steap3 | BC037435 | AGGTGACACTATAGAATACTCTCTGTGCAGTCTCGCTG | GTACGACTCACTATAGGGATGCAGAGATGACGTTGAAGG |
Hmox1 | NM_010442 | AGGTGACACTATAGAATACCTCACTGGCAGGAAATCAT | GTACGACTCACTATAGGGACCAGAGTGTTCATTCGAGCA |
Fpn1 | AF226613 | AGGTGACACTATAGAATATGCCTTAGTTGTCCTTTGGG | GTACGACTCACTATAGGGAGTGGAGAGAGAGTGGCCAAG |
Hamp1 | NM_032541 | AGGTGACACTATAGAATAGAGAGACACCAACTTCCCCA | GTACGACTCACTATAGGGATCAGGATGTGGCTCTAGGCT |
Ftl1 | NM_010240 | AGGTGACACTATAGAATAAAGATGGGCAACCATCTGAC | GTACGACTCACTATAGGGAGCCTCCTAGTCGTGCTTGAG |
Fth1 | NM_010239 | AGGTGACACTATAGAATACTCATGAGGAGAGGGAGCAT | GTACGACTCACTATAGGGAGTGCACACTCCATTGCATTC |