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Table 2 Oligonucleotide probes and hybridisation conditions used in the analysis of faecal bacteria by FISH and FCM

From: Intestinal dysbiosis and reduced immunoglobulin-coated bacteria associated with coeliac disease in children

Probe Target Bacterial group Sequence (5'-3') Hybridisation Conditions (°C) References
Eub 338 Domain bacteria GCT GCC TCC CGT AGG AGT 50 [13]
Non 338 Negative control ACA TCC TAC GGG AGG C 50 [14]
Bif 164 Bifidobacterium CAT CCG GCA TTA CCA CCC 50 [24]
Lab 158 Lactobacillus/Enterococcus GGT ATT AGC A(C/T)C TGT TTC CA 45 [25]
Bac 303 Bacteroides/Prevotella CCA ATG TGG GGG ACC TT 45 [26]
Ecol 1513 Escherichia coli CAC CGT AGT GCC TCG TCA TCA 50 [27]
Chis 150 Clostridium histolyticum TTA TGC GGT ATT AAT CT(C/T) CCT TT 50 [28]
C Lis 135 Clostridium lituseburense GTT ATC CGT GTG TAC AGG G 50 [28]
FPrau 645 Faecalibacterium prausnitzii CCT CTG CAC TAC TCA AGA AAA AC 50 [29]
SRB 687 Sulphate-reducing bacteria TAC GGA TTT CAC TCC T 50 [30]
STA Staphylococcus TCC TCC ATA TCT CTG CGC 50 [31]