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Table 2 Bacterial strains and plasmids used in this study.

From: Characterization of mleR, a positive regulator of malolactic fermentation and part of the acid tolerance response in Streptococcus mutans

Strain/plasmid Relevant Characteristicsa Reference/source
Strains   
   E. coli   
DH5α General cloning strain  
Tuner(DE3) Expression strain Novagen
   S. mutans   ATCC 700610
UA159 Wild-type, Erms, Sps This study
ALSM3 UA159ΔmleR, Ermr This study
ALSM20 UA159::ϕ(mleRP-luc), Spr This study
ALSM13 UA159ΔmleR::ϕ(mleRP-luc), Ermr, Spr This study
ALSM33 UA159::ϕ(mleSP-luc), Spr This study
ALSM34 UA159ΔmleR::ϕ(mleSP-luc), Ermr, Spr This study
   This study
   This study
Plasmids   
   pFW5 Suicide vector, Spr A. Podbielski [29]
   pHL222 Apr, luc H. Lössner
   pALEC15 Derivate pFW5, luc, Spr This study
   pALEC16 pALEC15 + ϕ(mleRP-luc), Spr This study
   pALEC47 pALEC15 + ϕ(mleSP-luc), Spr This study
  1. a Apr, ampicillin resistance; Spr, spectinomycin resistance; Ermr, erythromycin resistance; luc, luciferase.
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BMC Microbiology

ISSN: 1471-2180

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