|
L-malate concentration [mg/ml]
|
pH-value
|
---|
Time
|
WT
|
Δ mleR
|
WT
|
Δ mleR
|
---|
0 min
|
5.53
|
5.63
|
6.4
|
6.34
|
20 min
|
4.87
|
5.61
|
6.7
|
6.32
|
40 min
|
2.77
|
5.59
|
6.9
|
6.43
|
60 min
|
2.34
|
5.42
|
7.2
|
6.52
|
12 hours
|
1.26
|
3.51
|
8.2
|
7.32
|
- The capability to carry out malolactic fermentation was determined by measuring the L-malate concentration and the pH of the supernatant of cultures grown to late exponential phase (OD ~1.3). The values represent the average of two independent experiments. The standard deviation was less than 5%.