Strain
|
aoxB expression
ratio
+As(III)/-As(III)
|
Standard
error
|
---|
Wild type
|
9.406
|
0.630
|
Ha3109 (rpoN)
|
0.250
|
0.060
|
Ha483 (aoxR)
|
0.111
|
0.024
|
Ha482 (aoxS)
|
0.200
|
0.029
|
Ha2646 (dnaJ)
|
1.156
|
0.289
|
- Expression ratios of aoxB in H. arsenicoxydans wild-type and mutant strains without As(III) versus an As(III) 8 hours induction (1.33 mM), as measured by quantitative RT-PCR. Expression of each gene was normalized to the expression of the two housekeeping genes HEAR0118 and HEAR2922 coding for a peptide deformylase and a putative RNA methyltransferase, respectively. Standard errors were calculated from the data of two quantitative PCR replicates obtained from two independent biological replicates. Bold text indicates statistically significant induction.