Multiple controls affect arsenite oxidase gene expression in Herminiimonas arsenicoxydans

BMC Microbiology

Table 2 aoxB relative expression in H. arsenicoxydans wild-type and mutant strains.

Strain	aoxB expression ratio +As(III)/-As(III)	Standard error
Wild type	9.406	0.630
Ha3109 (rpoN)	0.250	0.060
Ha483 (aoxR)	0.111	0.024
Ha482 (aoxS)	0.200	0.029
Ha2646 (dnaJ)	1.156	0.289

Expression ratios of aoxB in H. arsenicoxydans wild-type and mutant strains without As(III) versus an As(III) 8 hours induction (1.33 mM), as measured by quantitative RT-PCR. Expression of each gene was normalized to the expression of the two housekeeping genes HEAR0118 and HEAR2922 coding for a peptide deformylase and a putative RNA methyltransferase, respectively. Standard errors were calculated from the data of two quantitative PCR replicates obtained from two independent biological replicates. Bold text indicates statistically significant induction.

ISSN: 1471-2180