Determination of aoxA transcription start site by 5'RACE and identification of a σ54 consensus motif. The transcription start site (TSS) of aoxA is in bold and indicated as +1 in the aoxA promoter sequence. The -12 and -24 boxes are highlighted and the consensus sequence is indicated in bold. The aoxA promoter was also aligned with the promoter sequences of A. tumefaciens, Thiomonas sp., Rhizobium sp. NT-26, Achromobacter sp., R. ferrireducens, O. tritici, C. aurantiacus, C. limicola, T. thermophilus, B. multivorans, R. litoralis, Pseudomonas sp.TS44, C. phaeobacteroides and C. aggregans. Two distincts sequences were shown A. DNA sequences with a σ54-dependent promoter motif (indicated in boxes). B. DNA sequences without a σ54-dependent promoter motif. Sequence informations of other genes were obtained from GenBank database and their localization on the chromosome or the plasmid is given by a nucleotide numbering. Their accession numbers are: A. tumefaciens (ABB51929.1), Thiomonas sp. (ABY19317.1), Rhizobium sp. NT-26 (AAR05655.1), Achromobacter sp. (ABP63659.1), R. ferrireducens (YP_524326.1), O. tritici (ACK38266.1), C. aurantiacus (YP_001634828.1), C. limicola (YP_001942455.1), T. thermophilus (YP_145367.1), B. multivorans (YP_001585660.1, YP_001941631.1, YP_001941634.1, YP_001585641.1), R. litoralis (ZP_02142508.1), Pseudomonas sp.TS44 (ACB05952.1), C. phaeobacteroides (YP_001960746.1) and C. aggregans (YP_002461760.1).