Toxicity of unsaturated fatty acids to the biohydrogenating ruminal bacterium, Butyrivibrio fibrisolvens

BMC Microbiology

Table 1 Effects of fatty acids and methyl esters (50 μg ml^-1) on growth of, and metabolism of fatty acids by, Butyrivibrio fibrisolvens JW11 in M2 medium.

	DHA	EPA	γ-LNA	LNA	LA	CLA	VA	OA	SA
Free fatty acids
Lag phase (h)
Mean	>72	>72	>72	37.0	7.1	4.7	0.01	1.88	0.82
SD				5.25	0.60	0.20	0.13	0.23	0.48
Biohydrogenation	No	No	Yes	Yes	Yes	Yes	No	No	No
End product at 72 h	ND^a	ND	Conjugated 18:3	VA	VA	VA	ND	ND	ND
Methyl esters
Lag phase (h)
Mean	NA	NA	NA	NA	0.37	-0.17	-0.23	-0.07	1.54
SD					1.12	0.44	0.35	0.09	0.23
Biohydrogenation	No	No	Yes	Yes	Yes	Yes	No	No	No
End product at 72 h	ND	ND	Conjugated 18:2	VA	VA	VA	ND	ND	ND

^aND - None detected
^bNA - Not analyzed. The first OD reading was made at 16 h, by which time the cultures had grown.

ISSN: 1471-2180