Figure 5
From: Genetic adaptation of Streptococcus mutans during biofilm formation on different types of surfaces
AI-2 signal secretion by S. mutans biofilms on different surfaces. Biofilms were grown on each material and the resulting conditioned media were exposed to V. harveyi MM77 for AI-2 bioassay. Fold induction in luminescence of each sample was calculated above background luminescence of the negative control (sample without addition of any conditioned medium) and was normalized by the value of total fluorescence of live bacteria within the relevant biofilm detected by CLSM.