Table 1 M. tuberculosis strains and plasmids
From: Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis
Strains/plasmids | Characteristics | Source |
|---|---|---|
E. coli DH5α |  | Invitrogen |
M. tuberculosis H37Rv | wild-type laboratory strain | ATCC 25618 |
FAME1 | M. tuberculosis suhBΔ | This study |
FAME2 | M. tuberculosis impAΔ | This study |
FAME4 | M. tuberculosis impCΔ::pFM96 | This study |
FAME7 | M. tuberculosis::pFM54 (impCΔ SCO) | This study |
FAME9 | FAME7 ::pFM96 | This study |
FAME11 | FAME7::pFM123 | This study |
FAME63 | FAME7::FM203 | This study |
FAME5 | M. tuberculosis ino1Δ | [23] |
FAME12 | M. tuberculosis ino1Δ::pFM54 (SCO) | This study |
FAME35 | M. tuberculosis::pFM151 (cysQΔ SCO) | This study |
FAME43 | FAME35::FM164 | This study |
FAME53 | cysQΔ::FM164 | This study |
FAME87 | FAME35::FM203 | This study |
FAME93 | cysQΔ::FM203 | This study |
FAME 120 | M. tuberculosis cysQΔ:: pUC-Hyg-int | This study |
pBluescript II SK+ | Â | Stratagene |
pGEM5 | Â | Promega |
pUC-Gm-int | pUC-based plasmid with Hin dIII cassette carrying gm and L5 int | [54] |
pUC-Hyg-int | pUC-based plasmid with Hin dIII cassette carrying hyg and L5 int | [54] |
p2NIL | gene manipulation vector, kan | [26] |
pGOAL19 | hyg pAg85-lacZ sacB Pac I cassette vector | [26] |
pIMP50 | pGEM5::impA | This study |
pIMP51 | pGEM5::impAΔ (Sph I 200 bp) | This study |
pIMP57 | p2NIL::impAΔ (Sph I 200 bp) | This study |
pFM74 | p2NIL::impAΔ (769 bp) | This study |
pFM75 | pFM74 with Pac I cassette of pGOAL19 | This study |
pFM33 | p2NIL::suhB | This study |
pFM48 | pFM33::suhBΔ | This study |
pFM52 | pFM48 with Pac I cassette of pGOAL19 | This study |
pFM31 | p2NIL::impC | This study |
pFM53 | pFM31::impCΔ | This study |
pFM54 | pFM53 with Pac I cassette of pGOAL19 | This study |
pFM94 | pBluescript SK+::impC (+288 bp upstream) | This study |
pFM96 | pFM94::int gm | This study |
pFM123 | pFM96::impC D86N | This study |
PMN013 | plasmid carrying the M. smegmatis porin gene mspA | [44] |
pFM203 | pMN013::int gm | This study |
pFM145 | p2NIL::cysQ | This study |
pFM148 | pFM145::cysQΔ | This study |
pFM151 | pFM148 with Pac I cassette of pGOAL19 | This study |
pFM160 | pBluescript SK+::cysQ (+352 bp upstream) | This study |
pFM164 | pFM160::int gm | This study |