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Table 1 M. tuberculosis strains and plasmids

From: Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis

Strains/plasmids Characteristics Source
E. coli DH5α   Invitrogen
M. tuberculosis H37Rv wild-type laboratory strain ATCC 25618
FAME1 M. tuberculosis suhBΔ This study
FAME2 M. tuberculosis impAΔ This study
FAME4 M. tuberculosis impCΔ::pFM96 This study
FAME7 M. tuberculosis::pFM54 (impCΔ SCO) This study
FAME9 FAME7 ::pFM96 This study
FAME11 FAME7::pFM123 This study
FAME63 FAME7::FM203 This study
FAME5 M. tuberculosis ino1Δ [23]
FAME12 M. tuberculosis ino1Δ::pFM54 (SCO) This study
FAME35 M. tuberculosis::pFM151 (cysQΔ SCO) This study
FAME43 FAME35::FM164 This study
FAME53 cysQΔ::FM164 This study
FAME87 FAME35::FM203 This study
FAME93 cysQΔ::FM203 This study
FAME 120 M. tuberculosis cysQΔ:: pUC-Hyg-int This study
pBluescript II SK+   Stratagene
pGEM5   Promega
pUC-Gm-int pUC-based plasmid with Hin dIII cassette carrying gm and L5 int [54]
pUC-Hyg-int pUC-based plasmid with Hin dIII cassette carrying hyg and L5 int [54]
p2NIL gene manipulation vector, kan [26]
pGOAL19 hyg pAg85-lacZ sacB Pac I cassette vector [26]
pIMP50 pGEM5::impA This study
pIMP51 pGEM5::impAΔ (Sph I 200 bp) This study
pIMP57 p2NIL::impAΔ (Sph I 200 bp) This study
pFM74 p2NIL::impAΔ (769 bp) This study
pFM75 pFM74 with Pac I cassette of pGOAL19 This study
pFM33 p2NIL::suhB This study
pFM48 pFM33::suhBΔ This study
pFM52 pFM48 with Pac I cassette of pGOAL19 This study
pFM31 p2NIL::impC This study
pFM53 pFM31::impCΔ This study
pFM54 pFM53 with Pac I cassette of pGOAL19 This study
pFM94 pBluescript SK+::impC (+288 bp upstream) This study
pFM96 pFM94::int gm This study
pFM123 pFM96::impC D86N This study
PMN013 plasmid carrying the M. smegmatis porin gene mspA [44]
pFM203 pMN013::int gm This study
pFM145 p2NIL::cysQ This study
pFM148 pFM145::cysQΔ This study
pFM151 pFM148 with Pac I cassette of pGOAL19 This study
pFM160 pBluescript SK+::cysQ (+352 bp upstream) This study
pFM164 pFM160::int gm This study