Figure 5

Relative expression of IL-1β , IL-6 and IL-8 genes in human gingival fibroblasts (HGF1) infected with P. gingivalis W83 and the epsC mutant. After a 6-hour challenge with P. gingivalis cells at MOI 1000:1 or 10.000:1 as indicated on the Y-axis, the expression levels of IL-1β, IL-6 and IL-8 in human gingival fibroblasts were measured using RT-PCR and represented as a relative value compared to a non-infected control sample which is set to a value of 1. Significant differences p < 0.01 are indicated by an asterisk.