| | MEST-1 Galf β1→3/6Manp | MEST-2 GlcCer | MEST-3 Manp α1→3Manp α1→2Ins |
---|
| |
HPTLC
|
IFI
|
HPTLC
|
IFI
|
HPTLC
|
IFI
|
Pb | Y |
+
|
+
|
+
|
+
|
+
|
+
|
| M |
+
|
-
|
+
|
-
|
+
|
-
|
Ss | Y | - (np) | - (np) |
+
|
+
|
+
|
+
|
| M | - (np) | - (np) |
+
|
-
| - (np) | - (np) |
Hc | Y |
+
|
+
|
+
|
+
|
+
|
+
|
| M | - (np) | - (np) |
+
|
-
| - (np) | - (np) |
- Reactivity of mAbs MEST-1, -2 and -3, with fungal glycolipids by HPTLC immunostaining (HPTLC); and with fixed fungi by indirect immunofluorescence (IFI). Pb = P. brasiliensis; Ss = S. schenckii; Hc = H. capsulatum; Y = yeast; M = mycelium; MEST-1 recognizes epitope Galf β1→3/6Manp; MEST-2 recognizes fungal glucosylceramide (GlcCer); MEST-3 recognizes epitope Manp α1→3Manp α1→2Ins; "+" indicates positive staining; "-" indicates negative staining, and "np" indicates epitope not present. [13, 24].