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Table 1 Reactivity of mAbs MEST-1, -2 and -3, with different fungi preparation

From: Effect of anti-glycosphingolipid monoclonal antibodies in pathogenic fungal growth and differentiation. Characterization of monoclonal antibody MEST-3 directed to Manp α1→3Manp α1→2IPC

  

MEST-1

Galf β1→3/6Manp

MEST-2

GlcCer

MEST-3

Manp α1→3Manp α1→2Ins

  

HPTLC

IFI

HPTLC

IFI

HPTLC

IFI

Pb

Y

+

+

+

+

+

+

 

M

+

-

+

-

+

-

Ss

Y

- (np)

- (np)

+

+

+

+

 

M

- (np)

- (np)

+

-

- (np)

- (np)

Hc

Y

+

+

+

+

+

+

 

M

- (np)

- (np)

+

-

- (np)

- (np)

  1. Reactivity of mAbs MEST-1, -2 and -3, with fungal glycolipids by HPTLC immunostaining (HPTLC); and with fixed fungi by indirect immunofluorescence (IFI). Pb = P. brasiliensis; Ss = S. schenckii; Hc = H. capsulatum; Y = yeast; M = mycelium; MEST-1 recognizes epitope Galf β1→3/6Manp; MEST-2 recognizes fungal glucosylceramide (GlcCer); MEST-3 recognizes epitope Manp α1→3Manp α1→2Ins; "+" indicates positive staining; "-" indicates negative staining, and "np" indicates epitope not present. [13, 24].