Figure 4
From: An enhancer peptide for membrane-disrupting antimicrobial peptides
Effect of NP4P on the membrane-disrupting activity of ASABF-α against the cytoplasmic membrane of S. aureus. Disruption of the cytoplasmic membrane was estimated by the increase in fluorescence intensity of diS-C3-(5). Changes in fluorescence were normalized by the value at the plateau of the dose-response curves. (A) Dose-response curve and (B) dose-bactericidal effect curve of ASABF-α against S. aureus IFO12732. These curves were simultaneously determined. The asterisks indicate that viable cells were not detected. (C) Effect of NP4P on the cytoplasmic membrane. The time courses of fluorescence changes are represented. (D) Effect of NP4P on cytoplasmic membrane disruption by ASABF-α. Dose-response curves were determined in the presence of NP4P at various concentrations (0, 30, and 100 μg/ml). (E) Another assay for NP4P enhancement. NP4P was applied after treatment of 1.28 μg/mL of ASABF-α. The fluorescent change evoked only by ASABF-α is indicated by a dashed line.