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Figure 3 | BMC Microbiology

Figure 3

From: Identification of genes involved in the mutualistic colonization of the nematode Heterorhabditis bacteriophora by the bacterium Photorhabdus luminescens

Figure 3

Attachment to abiotic surface by P. luminescens. Photorhabdus strains (as indicated) were grown overnight at 30°C in LB broth (+ Km). The OD600 of the culture was adjusted to 0.05 and 200 μl was added to the well of a 96-well Costar® PP microtitre plate. The plates were incubated for 72 h at 30°C before staining with crystal violet to quantify bacterial attachment. Relative biofilm formation was determined by calculating the OD595 (mutant):OD595 (TT01gfp) ratio and the results shown are the mean ± SD of 3 experiments.

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