Table 1 Proteins identified in the first trypsin digest with and without a sodium carbonate wash step.
Protein type | Sample Group | |||
|---|---|---|---|---|
| Â | HPLC grade water wash | Sodium Carbonate wash | ||
| Â | Incl 1 peptide | >1 peptide | Incl 1 peptide | >1 peptide |
All types | 301 | 198 | 233 | 142 |
Non-membrane | 253 | 168 | 134 | 81 |
Membrane-associated | 48 | 30 | 99 | 61 |
OMP | 26 | 14 | 54 | 42 |
% Non-membrane | 84% | 85% | 58% | 57% |
% Membrane-assoc. | 16% | 15% | 42% | 43% |
% OMP | 9% | 7% | 23% | 29% |