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Figure 7 | BMC Microbiology

Figure 7

From: Identification and functional characterisation of Complement Regulator Acquiring Surface Protein-1 of serum resistant Borrelia garinii OspA serotype 4

Figure 7

Mapping of the binding domains of CFH and FHL-1 for BGA66 and BGA71. Schematic representation of the CFH and FHL-1 protein and ligand affininty blot analysis of fusion proteins. The complement regulatory domains SCR 1-4 are in checked. Purified recombinant protein was separated by 10% Tris-Tricine-SDS-PAGE and transferred to nitrocellulose. Membranes were incubated with either recombinant FHL-1 (FH1-7) or several deletion constructs of CFH (FH1-2, FH1-3, FH1-4, FH1-5, FH1-6, FH8-20) or with human serum (FH). Bound proteins were visualized using polyclonal goat anti-CFH (Calbiochem), or MAb VIG8 directed against the C-terminus of CFH. SCR 5-7 are essential SCR for binding of BGA66 and BGA71 to interact with CFH/FHL-1.

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