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Figure 5 | BMC Microbiology

Figure 5

From: Identification and functional characterisation of Complement Regulator Acquiring Surface Protein-1 of serum resistant Borrelia garinii OspA serotype 4

Figure 5

Binding capabilities of CFH and FHL-1 to CspA orthologs of B. garinii ST4. Purified GST fusion proteins, BbCspA, BGA66, BGA67, BGA69, and BGA71 (500 ng/lane) were subjected to 10% Tris/Tricine SDS-PAGE and blotted to nitrocellulose membranes. Membranes were then incubated with recombinant FHL-1 or with NHS. GST-fusion proteins were detected by using anti-goat GST antibody and binding to CFH and FHL-1 were visualized using mAb VIG8 specific for the C-terminal region of CFH and ╬▒SCR1-4 antiserum specific for the N-terminal region of FHL-1. Binding of CFH and FHL-1 is visible for BGA66 and BGA71.

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