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Table 2 LAMP and PCR primers used in this study to detect Vibrio parahaemolyticus

From: Development of a toxR-based loop-mediated isothermal amplification assay for detecting Vibrio parahaemolyticus

Primer name

Sequence (5'-3')

Positiona

Amplicon size (bp)

Reference

F3

TTGGATTCCACGCGTTAT

528-545

Ladder-like bands for LAMP; 183 bp for F3/B3 PCR

This study

B3

CGTTCAATGCACTGCTCA

693-710

  

FIP

TGAGATTCCGCAGGGTTTGTAA

TTATTTTTGGCACTATTACTACCG

587-608 (F1c)

547-570 (F2)

  

BIP

GTTCCGTCAGATTGGTGAGTATC

TAGAAGGCAACCAGTTGTT

609-631(B1c)

673-691(B2)

  

Loop

AGAACGTACCAGTGATGACACC

632-653

  

toxR-F

GTCTTCTGACGCAATCGTTG

453-472b

367b

[18]

toxR-R

ATACGAGTGGTTGCTGTCATG

799-819b

  
  1. aThe positions are numbered based on the coding sequence of V. parahaemolyticus strain AQ3815 toxR gene [GenBank: L11929].
  2. bDifferences in primer positions and amplicon size were noted from those originally published after reanalysis of the sequences.