Northern blot analysis of transcripts using total RNA. (A) Overexpression of genes encoding the NIMA interactive protein [GenBank: FE526568], FYVE protein [GenBank: FE526741], and aminoacid permease [GenBank: FE526515] in T. rubrum mycelia exposed to acidic pH for 30 min (Library 8). Lanes 1 and 2 represent the H6 strain incubated at pH 5.0 and pH 8.0 (control), respectively. (B)Overexpression of genes encoding hs p30 [GenBank: FE526362], NIMA interactive protein [GenBank: FE526568], and a no-match transcript [GenBank: FE526434] in T. rubrum grown in keratin for 72 h (Library 7). Lanes 1 and 2 represent the H6 strain cultured with keratin or glucose (control) as the carbon source, respectively. Ethidium-bromide-stained rRNA bands are shown to allow comparison of the quantities of loaded RNAs. Hybridization with the 18S rRNA gene was performed as an additional loading control for northern blots. Bars show fold expression, determined from the intensity measured by densitometric analysis.