Pseudomonas aeruginosa Cystic Fibrosis isolates of similar RAPD genotype exhibit diversity in biofilm forming ability in vitro

Table 2 Strains and plasmids used in this study.

Strain/plasmids	Genotype/phenotype	Source/reference
*E. coli* *E coli* JM109	End1 recA1 gyrA96 this hsdR17(r_k^-m_k⁺) relA1 supE44 Δlac-proAB (F' traD36 proAB lacI^qZΔAM15)	Promega
*P. aeruginosa* ATCC 15442		Centre for Biofilm Engineering, Montana
Plasmids
pRK2013	ColE1-Tra(RK2)⁺Km^r	Figurski & Helinski, (1979) [47]
pUX-BF13	R6 K replicon -based helper plasmid providing the Tn7 transposition function in trans. Ap^r, mob⁺	Bao et al. (1991) [48]
pBK-miniTn7-gfp3	pUC19 based delivery plasmid or miniTn7-gfp3. Km^r, Ap^r, Cm^r, Sm^r, mob⁺	Koch et al. (2001)

ISSN: 1471-2180