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Table 2 Strains and plasmids used in this study.

From: Pseudomonas aeruginosa Cystic Fibrosis isolates of similar RAPD genotype exhibit diversity in biofilm forming ability in vitro

Strain/plasmids Genotype/phenotype Source/reference
E. coli
E coli JM109
End1 recA1 gyrA96 this hsdR17(rk-mk+) relA1 supE44 Δlac-proAB (F' traD36 proAB lacIqZΔAM15) Promega
P. aeruginosa
ATCC 15442
  Centre for Biofilm Engineering, Montana
pRK2013 ColE1-Tra(RK2)+Kmr Figurski & Helinski, (1979) [47]
pUX-BF13 R6 K replicon -based helper plasmid providing the Tn7 transposition function in trans. Apr, mob+ Bao et al. (1991) [48]
pBK-miniTn7-gfp3 pUC19 based delivery plasmid or miniTn7-gfp3. Kmr, Apr, Cmr, Smr, mob+ Koch et al. (2001)