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Table 2 Primers for RT-PCR and Quantitative Real Time RT-PCR

From: Genome-wide investigation and functional characterization of the β-ketoadipate pathway in the nitrogen-fixing and root-associated bacterium Pseudomonas stutzeriA1501

Primer No.

Primer name

Sequence (5'-3')

Amplified fragmenta

1

RT1-5'

AGCGAGAACCAATGGC

782 bp benRA intergenic region

2

RT1-3'

TAGTCGATTCCCAGGG

 

3

RT2-5'

GCACTGGATCGAGGGAGC

456 bp benAB intergenic region

4

RT2-3'

GTTGTGCGAGGTGCGTGT

 

5

RT3-5'

GCTTTCGCTACAAGACCG

503 bp benBC intergenic region

6

RT3-3'

CGCACGTTGCTGATGGTC

 

7

RT4-5'

CGAACCCAAACACCTCAA

546 bp benCD intergenic region

8

RT4-3'

CTCGGCCTCGATCTCATG

 

9

RT5-5'

TACCAGGAACATGAGAT

610 bp benDK intergenic region

10

RT5-3'

ACGTCTACTTTTCGCATG

 

11

RT6-5'

GTTCTTCTGTTGCCTG

1074 bp benK and catB intergenic region

12

RT6-3'

TCTTCGATGTCCTTAG

 

13

RT7-5'

CCTTCGTCACCCTCAACA

309 bp catBC intergenic region

14

RT7-3'

CTTCACGCATCAGGCTCT

 

15

RT8-5'

GAAGATGATCGTGAAAC

1030 bp catCA intergenic region

16

RT8-3'

TGAAGAAATGAATGTGC

 

17

benA-5'

CGGCTCGTCCACCTATGTCTAT

186 bp internal fragment

18

benA-3'

AAACCACCGCCCTTCTTGC

 

19

catB-5'

CCTTCGTCACCCTCAACAG

159 bp internal fragment

20

catB-3'

TCCAGGCTCAGGCCAAGAC

 

21

pcaD-5'

TTCGCCGAGCATTTCCG

173 bp internal fragment

22

pcaD-3'

CCGATCAGTCCGCCCAT

 
  1. aPCR reactions were carried out with the sets of primers indicated to the left.