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Table 1 Strains and plasmids used in this study

From: Genome-wide investigation and functional characterization of the β-ketoadipate pathway in the nitrogen-fixing and root-associated bacterium Pseudomonas stutzeriA1501

Strains or plasmids Relevant characteristic(s)a Source or reference
Strains   
P. stutzeri   
A1501 Wild type, Chinese culture CGMCC 0351, Ben++, Cat++, 4HBA+ [22]
A1601 A1501 benR::pK18mob ΔbenR, Ben-, Cat++, 4HBA+ this study
A1602 A1501 pcaR::pK18mob ΔpcaR, Ben-, Cat-, 4HBA- this study
A1603 A1501 pcaD::pK18mob ΔpcaD, Ben-, Cat-, 4HBA- this study
A1608 A1601 harboring complement plasmid pLbenR this study
A1609 A1602 harboring complement plasmid pLpcaR this study
A1610 A1603 harboring complement plasmid pLpcaD this study
Plasmids   
pK18mob Kmr; oriColE1 Mob+ lacZα+, used for directed insertional disruption [45]
pRK2013 Kmr; Tra+, oriColE1 [46]
pLAFR3 Tcr; Tra-, Mob+, cos, RK2 replicon [47]
pKbenR Kmr; 293 bp EcoR I-Hind III fragment containing part of benR in pK18mob this study
pKpcaR Kmr; 299 bp EcoR I-Hind III fragment containing part of pcaR in pK18mob this study
pKpcaD Kmr; 361 bp EcoR I-Hind III fragment containing part of pcaD in pK18mob this study
pLbenR Tcr; 1041 bp EcoR I-Hind III fragment containing benR with its native promoter in pLAFR3 this study
pLpcaR Tcr; 1457 bp EcoR I-Hind III fragment containing pcaR with its native promoter in pLAFR3 this study
pLpcaD Tcr; 820 bp EcoR I-Hind III fragment containing pcaD with the lac promoter in pLAFR3 this study
  1. a Ben++, good growth on benzoate; Cat++, good growth on catechol; 4HBA+, weak growth on 4-hydroxybenzoate; Ben-, no growth on benzoate; Cat-, no growth on catechol; 4HBA-, no growth on 4-hydroxybenzoate; Kmr, kanamycin resistant; Tcr, tetracycline resistant.