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Figure 6 | BMC Microbiology

Figure 6

From: Genome-wide investigation and functional characterization of the β-ketoadipate pathway in the nitrogen-fixing and root-associated bacterium Pseudomonas stutzeriA1501

Figure 6

Induction of the benA or catB promoters in culture media with several different inducers. The putative binding site for BenR or CatR is boxed. The putative -10/-35 promoter consensus sequences are indicated by asterisks. The predicted transcriptional start site (+1) and ribosome-binding site (RBS) are underlined. (A) Nucleotide sequence of the benR-benA intergenic region of strain A1501. (B) Quantitative real-time RT-PCR analysis of relative benA expression level of the wild type (black bars) and benR mutant (gray bars) in the presence or absence of benzoate (BEN), catechol (CAT), cis, cis-muconate (CCM), and lactate (LAC). (C) Comparison of the catB promoter of strain A1501 with those of P. putida PRS2000, P. aeruginosa PAO1 and P. fluorescens pf-5. Dashes indicate gaps to obtain maximal homology. (D) Quantitative real-time RT-PCR analysis of relative catB expression level of the wild type (black bars) and benR mutant (gray bars) in the presence or absence of benzoate (BEN), catechol (CAT), cis, cis-muconate (CCM), and lactate (LAC). Relative levels of transcripts are presented as the mean values ± SD, calculated from three sets of independent experiments.

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