Figure 4

Conversion of benzoate (BEN) to catechol (CAT) and cis, cis -muconate (CCM) by the pcaD mutant A1603. Cells were grown for 48 h in minimal medium supplemented with 4 mM benzoate. The elution profile of compounds separated by HPLC is shown. Accumulations of the intermediates catechol and cis, cis-muconate are indicated by red vertical arrows.