Figure 3

Complementation of hyperlethal phenotype by cloned genes. Plasmids containing wild-type genes were transformed into the corresponding Tn5-containing mutants. The strains harboring the plasmids were then tested for nalidixic acid-mediated lethality by treating mid-log phase cells with various concentrations of nalidixic acid for 2 hr at 37°C. Percent of control indicates percent survival of treated cells relative to untreated cells sampled at the time of drug addition. For ycjW, yrbB, and ybcM, the expression was induced by adding 1 mM of IPTG 2 hr before nalidixic acid treatment. Similar results were obtained in a replicate experiment.