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Figure 5 | BMC Microbiology

Figure 5

From: Staphylococcus aureus sigma B-dependent emergence of small-colony variants and biofilm production following exposure to Pseudomonas aeruginosa 4-hydroxy-2-heptylquinoline-N- oxide

Figure 5

P. aeruginosa stimulates biofilm formation and increases the activity of SigB of a S. aureus CF isolate. (A) CFU/ml recovered after 48 h of growth of CF1A-L (open bar) and CF1A-L in the presence of supernatants from overnight cultures of P. aeruginosa PAO1 (black bar) or of E. coli K12 (hatched bar). The picture shows the specific inhibitory effect of P. aeruginosa on the growth of S. aureus. (B) Relative biofilm production by CF1A-L grown in the presence of supernatants from overnight cultures of P. aeruginosa or E. coli. Pictures show the biofilm formation of CF1A-L in the absence or in the presence of culture supernatants of P. aeruginosa or E. coli as detected by crystal violet staining. (C) Relative number of SCV CFUs recovered after 6 h of growth for S. aureus CF1A-L in co-culture with PAO1 or K12 as determined using the double chamber co-culture model. (D) Relative expression ratios for the gene asp23 were evaluated by qPCR for CF1A-L in co-culture with PAO1 or K12. For B, C and D, results are normalized to unexposed CF1A-L (dotted line). Data are presented as means with standard deviations from three independent experiments. Significant differences between unexposed CF1A-L and the exposed conditions (*, P < 0.05; ***, P < 0.001) and between CF1A-L exposed to PAO1 or K12 (Δ, P < 0.05; ΔΔΔ, P < 0.001) were revealed by one-way ANOVA followed by the tuckey's post test.

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