p38 and ERK are involved in the stimulation of IL-8 secretion by V. parahaemolyticus. A: ELISA to detect secreted IL-8 6 h and 24 h after co-incubation with V. parahaemolyticus in presence of MAPK inhibitors. The experiment was performed essentially as outlined in Figure 5 with WT V. parahaemolyticus and the addition of MAPK inhibitors, SB203580 (5 μM), SP600125 (15 μM) or PD98059 (40 μM), as indicated. Results indicate mean ± SEM of three independent experiments. *P < 0.05 vs cells co-incubated with bacteria in absence of inhibitor.