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Figure 4 | BMC Microbiology

Figure 4

From: Pseudomonas aeruginosa β-lactamase induction requires two permeases, AmpG and AmpP

Figure 4

Topology of AmpP and AmpG. The topology of AmpP and AmpG was analyzed by in-frame ampP and ampG fusions to the lacZ and phoA genes, the cytoplasmic and periplasmic markers, respectively. The corresponding points of fusion and qualitative biochemical results of the β-galactosidase (LacZ) and alkaline phosphatase (PhoA) assays [44] are shown for AmpP (A) and AmpG (C). These results, together with transmembrane domain predictions generated using a Kyte-Doolittle algorithm present in Lasergene 7 (DNASTAR, Madison, WI) were used to predict the topology of AmpP (B) and AmpG (D). Solid lines indicate prediction based upon experimental data, dashed lines indicate regions where more than one possibility exists. Cytoplasm and periplasm are denoted by Cyto and Peri, respectively. Fusion sites are indicated by a dot with the corresponding amino acid number. Putative transmembrane domain boundaries were obtained from Lasergene.

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