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Figure 3 | BMC Microbiology

Figure 3

From: Pseudomonas aeruginosa β-lactamase induction requires two permeases, AmpG and AmpP

Figure 3

PCR analysis of ampFG and ampOP operon cDNA. Polyacrylamide gel electrophoresis of PCR products of the junctions of the ampOP and ampFG operons. (A) PCR with primers PA4392_3 junctionRTF and PA4392_3 junctionRTR to amplify the PA4392 - PA4393 intergenic region. (B) PCR with primers PA4218_9 junctionRTF and PA4218_9 junctionRTR to amplify the PA4392 - PA4393 intergenic region. (Panels A and B) Lane M: PCR markers (Promega, Madison, WI). Lane 1, cDNA reaction performed with PAO1 RNA, the appropriate buffer and Superscript RT III. Lane 2, cDNA reaction performed with PAO1 RNA, the appropriate buffer without Superscript RT III. Lane 3, P. aeruginosa genomic DNA. The asterisk indicates a nonspecific product. Arrows indicate junction amplicons.

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