Table 3 Primers
Name | Nucleotide sequence (5' to 3')a |
|---|---|
Construction of the deletion mutants | |
relA-FW | CGCCATCCCGCAATGGTTTACATAA |
relA-RV | TCATTGTTCTGGCCATAACAGC |
spoT-FW | CTTGAAAACCATCATTCGCGCTGAACG |
spoT-RV | TCTGCGGTACGAATGATTGCAGAAACG |
stm3169-red-FW | ACGTTCATTCACAACATCAGCGGTATTACTGGCCGGCTGTGTGTAGGCTGGAGCTGCTTC |
stm3169-red-RW | ACATATTCTCGATGTATTCCAGATCCTTCGCTGACTGAGCCATATGAATATCCTCCTTAG |
sseF-red-FW | AACAGAACGAAATATGAAAATTCATATTCCGTCAGCGGCAGTGTAGGCTGGAGCTGCTTC |
sseF-red-RW | TGTCCATTAATGCAGGTGTAGTAGCAGATTGACAGAGCGCCATATGAATATCCTCCTTAG |
pAC-tet-FW | TTGGTAGCTCAGAGAACCTTCGAAAAACCG |
pAC-tet-RV | TCGCTCGCGTATCGGTGATTCATTCGCTA |
Construction of plasmids for the complementations | |
relA-FW2 | AGGCTCGAGGTCGCGGTAAGAAGT |
relA-RV2 | ACAAGCTTACTGTCTGGGGTTTAC |
ssrB-FW | GGGCTCGAG GAATATAAGATCTTATTAGTA |
ssrB-RV | CCCGGATCC ATACTCTATTAACCTCATTCT |
stm3169-FW | CCGCTCGAG AACACACGTTCATTCACAACATCAG |
stm3169-RV | GGAAGATCT ATTCTCGATGTATTCCAGATCCTTC |
Construcion of the lacZ fusions | |
ssaG-Pro-FW | AAAGTCGAC CAAATGCTCAGGTAGGAGGGC |
ssaG-Pro-RV | AAAGGATCC ATCATCGATTCTGGGTTGAGC |
stm3169-Pro-FW | ACGCGTCGAC GACGATTTAGCCGGTATGAAAATCA |
stm3169-Pro-RV | CGGGATCC TTACATATTCTCGATGTATTCCAGA |
Comfirmation of gene expression by qRT-PCR | |
gyrA-FW | AAGAGCTCCTATCTGGATTATGC |
gyrA-RV | TATTTACCGATTACGTCACCAAC |
relA-FW | ATTGTGCCATTCACCTATCAGTT |
relA-RV | GATATTTTTGTCACGATCCTGCT |
invF-FW | ATCGCTGCTGAATAGTGTAGAAG |
invF-RV | CATTTGTCTGCCAATTGAATAAT |
stm0209-FW | CCTGAACGTAGAAAATTACGAGA |
stm0209-RV | GTCAGGTTTTTCACCATGTTACT |
stm0435-FW | GTCAATCAGTTGCTCGATATTCTG |
stm0435-RV | TTTAATCAGCTTGACGATTTTCTTC |
stm0748-FW | TGAACCTGTACGTTATGGATCTC |
stm0748-RV | CGCCGTTAATGTTCATTTTATAC |
stm0781-FW | GAAGGCAAGATCACCGTATTT |
stm0781-RV | CTGATCAGCAGAGATGAAGAGAT |
stm1478-FW | ACAAAAGTTGAGGAGCTGAATAAAG |
stm1478-RV | GCCACTGACGCGTAATATGATAA |
stm1720-FW | TTGGTTGTAAAATTGAAGACAAAGG |
stm1720-RV | GTCCCCTTCAGGATAAAGGTGTAAT |
stm1746-FW | CGAATTATTCCAGAAACTGAAGAAA |
stm1746-RV | ATCGCCCTGATTTTTAACCTTATTA |
stm2638-FW | TATTCTGACGGTCTGTTTAGCTTTT |
stm2638-RV | GTACTGCCCTGAATTTGATACTGTC |
stm3169-FW | GTTACCAGAATAATGTCGCAGCTAT |
stm3169-RV | AATCATCCACATAAAAAGAATCTGG |
stm3318-FW | CAAACTCAGCCTTAATCTTATGC |
stm3318-RV | ACTTTATCGGCGTTGATCTTAAT |
stm4319-FW | ATTAGTATTATCCGAGGCCAGAC |
stm4319-RV | CAGTCTTGCAAACTCTACTGCTC |
stm4403-FW | ATTGATATTCACAGCAACAAACC |
stm4403-RV | AGGTCAGGTTTTTAATACGTTCC |
stm4405-FW | CGAACTGACGTTGAATAAAGATGAG |
stm4405-RV | AATTGTGGTCGTCTGGTATCTGT |