Table 2 Bacterial strains and plasmids used.
Strains | Relevant characteristics | Source/Ref. |
|---|---|---|
Bacterial strains S. Typhimurium | Â | |
14028 | wild-type | ATCC |
SH100 | Spontaneous nalidixic acid resistant derivative of wild-type 14028 | [44] |
TM157 | SH100 ΔrelA::cat ΔspoT::kan | this study |
YY2 | SH100 ΔrelA::cat ΔspoT::kan ΔssrB::tet | this study |
TH973 | SH100 Δstm3169::kan | this study |
TH1162 | SH100 stm3169::lacZ | this study |
TH1164 | TM157 stm3169::lacZ | this study |
YY3 | TH1164 ΔssrB::tet | this study |
TM129 | SH100 ssaG::lacZ | this study |
YY1 | SH100 ΔssrB::tet | this study |
SH113 | SH100 ΔssaV::cat | [11] |
TM548 | SH100 ΔsseF::kan | this study |
E. coli | Â | Â |
DH5α | K-12 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 (lacXYA-argR)U169 deoR (80 dlac (lacZ)M15) | Invirtogen |
SM10λpir | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpir | [45] |
Plasmids | Â | Â |
pGEM-Teasy | TA cloning vector | Promega |
pMW118 | pSC101-based low copy number plasmid | Nippon Gene |
pACYC184 | p15A-based low copy number plasmid, tet template | New England Biolabs |
pFLAG-CTC | FLAG tag expression vector | Sigma |
pLD-lacZ Ω | Integrational plasmid with promoterless lacZ gene | [39] |
pBAD-HisA | Expression vector for His6 fusion protein | Invitrogen |
pMW-Stm3169 | stm3169 gene in pMW118 | this study |
pLD-stm3169Z | stm3169::lacZ operon fusion in pLD-lacZ Ω | this study |
pLD-ssaGZ | ssaG::lacZ operon fusion in pLD-lacZ Ω | this study |
pRelA | pBAD-HisA expressing relA gene | this study |
pSsrB | pFLAG-CTC expressing ssrB gene | this study |
pKD46 | Red recombinase expression plasmid | [41] |
pKD4 | kan cassette used for gene deletion | [41] |