Ectopic expression of Scl1 on E. coli. (A) FACS analysis on whole bacteria pre-incubated with (white profile) or without (gray profile) anti-Scl1 antibodies, followed by FITC-conjugated secondary antibodies. (B) Electron microscope view of whole bacteria after negative staining with sodium phosphotungstate. Asterisks indicate ectopic expressed Scl1 on the E. coli surface. Bars represent 100 nm. ET2, E. coli expressing vector only. ET3, E. coli expressing Scl1. (C) Western blot analysis with anti-Scl1 (left panel) and anti-OmpA (right panel) antibodies in the outer membrane fraction of ET2 and ET3.