Expression profile and adhesion ability of scl1 -mutated S. pyogenes. (A) mRNA levels in fibronectin binding proteins (sfb and prtF1), olidopeptidase A (oppA), streptococcal collagen-like proteins (scl1 and scl2), and exotoxin B (speB) as an expression control. (B) HEp-2 cells were incubated with FITC-conjugated wild-type (WT) and Scl1-mutated S. pyogenes (ST2). The adhesion ability is expressed as the ratio of florescence from adherent bacteria to that from inoculated bacteria. Data represent means of five experiments with triplicate samples in each experiment. **, P < 0.01 compared with S. pyogenes wild-type M29588 strain.